/* reheader.c -- reheader subcommand. Copyright (C) 2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include #include #include #include #include #include #include #include #include "bcftools.h" #include "khash_str2str.h" typedef struct _args_t { char **argv, *fname, *samples_fname, *header_fname, *output_fname; htsFile *fp; htsFormat type; int argc; } args_t; static void read_header_file(char *fname, kstring_t *hdr) { kstring_t tmp = {0,0,0}; hdr->l = 0; htsFile *fp = hts_open(fname, "r"); if ( !fp ) error("Could not read: %s\n", fname); while ( hts_getline(fp, KS_SEP_LINE, &tmp) > 0 ) { kputsn(tmp.s,tmp.l,hdr); kputc('\n',hdr); } if ( hts_close(fp) ) error("Close failed: %s\n", fname); free(tmp.s); while ( hdr->l>0 && isspace(hdr->s[hdr->l-1]) ) hdr->l--; // remove trailing newlines kputc('\n',hdr); } static int set_sample_pairs(char **samples, int nsamples, kstring_t *hdr, int idx) { int i, j, n; // Are these samples "old-name new-name" pairs? void *hash = khash_str2str_init(); for (i=0; il>0 && isspace(hdr->s[hdr->l-1]) ) hdr->l--; // remove trailing newlines hdr->s[hdr->l] = 0; kstring_t tmp = {0,0,0}; i = j = n = 0; while ( hdr->s[idx+i] && hdr->s[idx+i]) { if ( hdr->s[idx+i]=='\t' ) { hdr->s[idx+i] = 0; if ( ++n>9 ) { char *ori = khash_str2str_get(hash,hdr->s+idx+j); kputs(ori ? ori : hdr->s+idx+j, &tmp); } else kputs(hdr->s+idx+j, &tmp); kputc('\t',&tmp); j = ++i; continue; } i++; } char *ori = khash_str2str_get(hash,hdr->s+idx+j); kputs(ori ? ori : hdr->s+idx+j, &tmp); if ( hash ) khash_str2str_destroy(hash); hdr->l = idx; kputs(tmp.s, hdr); kputc('\n', hdr); free(tmp.s); return 1; } static void set_samples(char **samples, int nsamples, kstring_t *hdr) { // Find the beginning of the #CHROM line int i = hdr->l - 2, ncols = 0; while ( i>=0 && hdr->s[i]!='\n' ) { if ( hdr->s[i]=='\t' ) ncols++; i--; } if ( i<0 || strncmp(hdr->s+i+1,"#CHROM\tPOS\tID\tREF\tALT\tQUAL\tFILTER\tINFO\tFORMAT",45) ) error("Could not parse the header: %s\n", hdr->s); // Are the samples "old-sample new-sample" pairs? if ( set_sample_pairs(samples,nsamples,hdr, i+1) ) return; // Replace all samples if ( ncols!=nsamples+8 ) fprintf(stderr, "Warning: different number of samples: %d vs %d\n", nsamples,ncols-8); ncols = 0; while ( ncols!=9 ) { i++; if ( hdr->s[i]=='\t' ) ncols++; } hdr->l = i; for (i=0; ifp); if ( !fp || bgzf_read_block(fp) != 0 || !fp->block_length ) error("Failed to read %s: %s\n", args->fname, strerror(errno)); kstring_t hdr = {0,0,0}; char *buffer = (char*) fp->uncompressed_block; // Read the header and find the position of the data block if ( buffer[0]!='#' ) error("Could not parse the header, expected '#', found '%c'\n", buffer[0]); int skip_until = 1; // end of the header in the current uncompressed block while (1) { if ( buffer[skip_until]=='\n' ) { skip_until++; if ( skip_until>=fp->block_length ) { kputsn(buffer,skip_until,&hdr); if ( bgzf_read_block(fp) != 0 || !fp->block_length ) error("FIXME: No body in the file: %s\n", args->fname); skip_until = 0; } // The header has finished if ( buffer[skip_until]!='#' ) { kputsn(buffer,skip_until,&hdr); break; } } skip_until++; if ( skip_until>=fp->block_length ) { kputsn(buffer,fp->block_length,&hdr); if (bgzf_read_block(fp) != 0 || !fp->block_length) error("FIXME: No body in the file: %s\n", args->fname); skip_until = 0; } } int nsamples = 0; char **samples = NULL; if ( args->samples_fname ) samples = hts_readlines(args->samples_fname, &nsamples); if ( args->header_fname ) { free(hdr.s); hdr.s = NULL; hdr.l = hdr.m = 0; read_header_file(args->header_fname, &hdr); } if ( samples ) { set_samples(samples, nsamples, &hdr); int i; for (i=0; ioutput_fname ) bgzf_out = bgzf_open(args->output_fname,"w"); else bgzf_out = bgzf_dopen(fileno(stdout), "w"); bgzf_write(bgzf_out, hdr.s, hdr.l); free(hdr.s); // Output all remainig data read with the header block if ( fp->block_length - skip_until > 0 ) { if ( bgzf_write(bgzf_out, buffer+skip_until, fp->block_length-skip_until)<0 ) error("Error: %d\n",fp->errcode); } if ( bgzf_flush(bgzf_out)<0 ) error("Error: %d\n",bgzf_out->errcode); // Stream the rest of the file without as it is, without decompressing ssize_t nread; int page_size = getpagesize(); char *buf = (char*) valloc(page_size); while (1) { nread = bgzf_raw_read(fp, buf, page_size); if ( nread<=0 ) break; int count = bgzf_raw_write(bgzf_out, buf, nread); if (count != nread) error("Write failed, wrote %d instead of %d bytes.\n", count,(int)nread); } if (bgzf_close(bgzf_out) < 0) error("Error: %d\n",bgzf_out->errcode); if (bgzf_close(fp) < 0) error("Error: %d\n",fp->errcode); free(buf); } static void reheader_vcf(args_t *args) { kstring_t hdr = {0,0,0}; htsFile *fp = args->fp; while ( hts_getline(fp, KS_SEP_LINE, &fp->line) >=0 ) { kputc('\n',&fp->line); // hts_getline eats the newline character if ( fp->line.s[0]!='#' ) break; kputsn(fp->line.s,fp->line.l,&hdr); } int nsamples = 0; char **samples = NULL; if ( args->samples_fname ) samples = hts_readlines(args->samples_fname, &nsamples); if ( args->header_fname ) { free(hdr.s); hdr.s = NULL; hdr.l = hdr.m = 0; read_header_file(args->header_fname, &hdr); } if ( samples ) { set_samples(samples, nsamples, &hdr); int i; for (i=0; ioutput_fname ? open(args->output_fname, O_WRONLY|O_CREAT|O_TRUNC, 0666) : STDOUT_FILENO; if ( out==-1 ) error("%s: %s\n", args->output_fname,strerror(errno)); if ( write(out, hdr.s, hdr.l)!=hdr.l ) error("Failed to write %d bytes\n", hdr.l); free(hdr.s); if ( fp->line.l ) { if ( write(out, fp->line.s, fp->line.l)!=fp->line.l ) error("Failed to write %d bytes\n", fp->line.l); } while ( hts_getline(fp, KS_SEP_LINE, &fp->line) >=0 ) // uncompressed file implies small size, we don't worry about speed { kputc('\n',&fp->line); if ( write(out, fp->line.s, fp->line.l)!=fp->line.l ) error("Failed to write %d bytes\n", fp->line.l); } hts_close(fp); close(out); } static bcf_hdr_t *strip_header(bcf_hdr_t *src, bcf_hdr_t *dst) { bcf_hrec_t *src_hrec, *dst_hrec, *tmp; bcf_hdr_t *out = bcf_hdr_init("r"); int i; for (i=0; inhrec; i++) { // first insert lines which do not code BCF ids, their order does not matter dst_hrec = dst->hrec[i]; if ( dst_hrec->type==BCF_HL_FLT || dst_hrec->type==BCF_HL_INFO || dst_hrec->type==BCF_HL_FMT || dst_hrec->type== BCF_HL_CTG ) continue; bcf_hdr_add_hrec(out, bcf_hrec_dup(dst_hrec)); } for (i=0; inhrec; i++) { // now transfer header lines which define BCF ids src_hrec = src->hrec[i]; if ( src_hrec->type==BCF_HL_FLT || src_hrec->type==BCF_HL_INFO || src_hrec->type==BCF_HL_FMT || src_hrec->type== BCF_HL_CTG ) { int j = bcf_hrec_find_key(src_hrec, "ID"); dst_hrec = bcf_hdr_get_hrec(dst, src_hrec->type, "ID", src_hrec->vals[j], NULL); if ( !dst_hrec ) continue; tmp = bcf_hrec_dup(dst_hrec); j = bcf_hrec_find_key(src_hrec, "IDX"); if ( j>=0 ) { j = atoi(src_hrec->vals[j]); hrec_add_idx(tmp, j); } bcf_hdr_add_hrec(out, tmp); } } bcf_hdr_sync(out); for (i=0; inhrec; i++) { // finally add new structured fields dst_hrec = dst->hrec[i]; if ( dst_hrec->type==BCF_HL_FLT || dst_hrec->type==BCF_HL_INFO || dst_hrec->type==BCF_HL_FMT || dst_hrec->type== BCF_HL_CTG ) { int j = bcf_hrec_find_key(dst_hrec, "ID"); tmp = bcf_hdr_get_hrec(out, dst_hrec->type, "ID", dst_hrec->vals[j], NULL); if ( !tmp ) bcf_hdr_add_hrec(out, bcf_hrec_dup(dst_hrec)); } } for (i=0; in[BCF_DT_SAMPLE]; i++) bcf_hdr_add_sample(out, dst->samples[i]); bcf_hdr_destroy(dst); return out; } static void reheader_bcf(args_t *args, int is_compressed) { htsFile *fp = args->fp; bcf_hdr_t *hdr = bcf_hdr_read(fp); if ( !hdr ) error("Failed to read the header: %s\n", args->fname); kstring_t htxt = {0,0,0}; int hlen; htxt.s = bcf_hdr_fmt_text(hdr, 1, &hlen); htxt.l = hlen; int i, nsamples = 0; char **samples = NULL; if ( args->samples_fname ) samples = hts_readlines(args->samples_fname, &nsamples); if ( args->header_fname ) { free(htxt.s); htxt.s = NULL; htxt.l = htxt.m = 0; read_header_file(args->header_fname, &htxt); } if ( samples ) { set_samples(samples, nsamples, &htxt); for (i=0; iheader_fname ) hdr_out = strip_header(hdr, hdr_out); // write the header and the body htsFile *fp_out = hts_open(args->output_fname ? args->output_fname : "-",is_compressed ? "wb" : "wbu"); if ( !fp_out ) error("%s: %s\n", args->output_fname ? args->output_fname : "-", strerror(errno)); bcf_hdr_write(fp_out, hdr_out); bcf1_t *rec = bcf_init(); while ( bcf_read(fp, hdr, rec)==0 ) { // sanity checking, this slows things down. Make it optional? bcf_unpack(rec, BCF_UN_ALL); if ( rec->rid >= hdr_out->n[BCF_DT_CTG] || strcmp(bcf_hdr_int2id(hdr,BCF_DT_CTG,rec->rid),bcf_hdr_int2id(hdr_out,BCF_DT_CTG,rec->rid)) ) error("The CHROM is not defined: \"%s\"\n", bcf_hdr_int2id(hdr,BCF_DT_CTG,rec->rid)); for (i=0; id.n_flt; i++) { int id = rec->d.flt[i]; if ( id >= hdr_out->n[BCF_DT_ID] ) break; if ( !bcf_hdr_idinfo_exists(hdr_out,BCF_HL_FLT,id) ) break; if ( strcmp(hdr->id[BCF_DT_ID][id].key,hdr_out->id[BCF_DT_ID][id].key) ) error("FIXME: Broken FILTER ids: %s vs %s\n", hdr->id[BCF_DT_ID][id].key,hdr_out->id[BCF_DT_ID][id].key); } if ( i!=rec->d.n_flt ) error("The FILTER is not defined: \"%s\"\n", bcf_hdr_int2id(hdr,BCF_DT_ID,rec->d.flt[i])); for (i=0; in_info; i++) { int id = rec->d.info[i].key; if ( id >= hdr_out->n[BCF_DT_ID] ) break; if ( !hdr_out->id[BCF_DT_ID][id].key ) break; if ( !bcf_hdr_idinfo_exists(hdr_out,BCF_HL_INFO,id) ) break; if ( strcmp(hdr->id[BCF_DT_ID][id].key,hdr_out->id[BCF_DT_ID][id].key) ) error("FIXME: Broken INFO ids: %s vs %s\n", hdr->id[BCF_DT_ID][id].key,hdr_out->id[BCF_DT_ID][id].key); } if ( i!=rec->n_info ) error("The INFO tag is not defined: \"%s\"\n", bcf_hdr_int2id(hdr,BCF_DT_ID,rec->d.info[i].key)); for (i=0; in_fmt; i++) { int id = rec->d.fmt[i].id; if ( id >= hdr_out->n[BCF_DT_ID] ) break; if ( !hdr_out->id[BCF_DT_ID][id].key ) break; if ( !bcf_hdr_idinfo_exists(hdr_out,BCF_HL_FMT,id) ) break; if ( strcmp(hdr->id[BCF_DT_ID][id].key,hdr_out->id[BCF_DT_ID][id].key) ) error("FIXME: Broken FORMAT ids: %s vs %s\n", hdr->id[BCF_DT_ID][id].key,hdr_out->id[BCF_DT_ID][id].key); } if ( i!=rec->n_fmt ) error("The FORMAT tag is not defined: \"%s\"\n", bcf_hdr_int2id(hdr,BCF_DT_ID,rec->d.fmt[i].id)); bcf_write(fp_out,hdr_out,rec); } bcf_destroy(rec); free(htxt.s); hts_close(fp_out); hts_close(fp); bcf_hdr_destroy(hdr_out); bcf_hdr_destroy(hdr); } static void usage(args_t *args) { fprintf(stderr, "\n"); fprintf(stderr, "About: Modify header of VCF/BCF files, change sample names.\n"); fprintf(stderr, "Usage: bcftools reheader [OPTIONS] \n"); fprintf(stderr, "\n"); fprintf(stderr, "Options:\n"); fprintf(stderr, " -h, --header new header\n"); fprintf(stderr, " -o, --output write output to a file [standard output]\n"); fprintf(stderr, " -s, --samples new sample names\n"); fprintf(stderr, "\n"); exit(1); } int main_reheader(int argc, char *argv[]) { int c; args_t *args = (args_t*) calloc(1,sizeof(args_t)); args->argc = argc; args->argv = argv; static struct option loptions[] = { {"output",1,0,'o'}, {"header",1,0,'h'}, {"samples",1,0,'s'}, {0,0,0,0} }; while ((c = getopt_long(argc, argv, "s:h:o:",loptions,NULL)) >= 0) { switch (c) { case 'o': args->output_fname = optarg; break; case 's': args->samples_fname = optarg; break; case 'h': args->header_fname = optarg; break; case '?': usage(args); default: error("Unknown argument: %s\n", optarg); } } if ( optind>=argc ) { if ( !isatty(fileno((FILE *)stdin)) ) args->fname = "-"; // reading from stdin else usage(args); } else args->fname = argv[optind]; if ( !args->samples_fname && !args->header_fname ) usage(args); if ( !args->fname ) usage(args); args->fp = hts_open(args->fname,"r"); if ( !args->fp ) error("Failed to open: %s\n", args->fname); args->type = *hts_get_format(args->fp); if ( args->type.format==vcf ) { if ( args->type.compression==bgzf || args->type.compression==gzip ) reheader_vcf_gz(args); else reheader_vcf(args); } else reheader_bcf(args, args->type.compression==bgzf || args->type.compression==gzip); free(args); return 0; }