#!/opt/chipster/tools/Python-2.7.12/bin/python ''' Summarizing mapping statistics of a BAM or SAM file. ''' #import built-in modules import os,sys if sys.version_info[0] != 2 or sys.version_info[1] != 7: print >>sys.stderr, "\nYou are using python" + str(sys.version_info[0]) + '.' + str(sys.version_info[1]) + " RSeQC needs python2.7!\n" sys.exit() import re import string from optparse import OptionParser import warnings import string import collections import math import sets from time import strftime #import third-party modules from bx.bitset import * from bx.bitset_builders import * from bx.intervals import * #import my own modules from qcmodule import SAM #changes to the paths #changing history to this module __author__ = "Liguo Wang" __copyright__ = "Copyleft." __credits__ = [] __license__ = "GPL" __version__="2.6.4" __maintainer__ = "Liguo Wang" __email__ = "wang.liguo@mayo.edu" __status__ = "Production" def main(): usage="%prog [options]" + '\n' + __doc__ + "\n" parser = OptionParser(usage,version="%prog " + __version__) parser.add_option("-i","--input-file",action="store",type="string",dest="input_file",help="Alignment file in BAM or SAM format.") parser.add_option("-q","--mapq",action="store",type="int",dest="map_qual",default=30,help="Minimum mapping quality (phred scaled) to determine \"uniquely mapped\" reads. default=%default") (options,args)=parser.parse_args() if not (options.input_file): parser.print_help() sys.exit(0) if not os.path.exists(options.input_file): print >>sys.stderr, '\n\n' + input_file + " does NOT exists" + '\n' sys.exit(0) obj = SAM.ParseBAM(options.input_file) obj.stat(q_cut = options.map_qual) if __name__ == '__main__': main()