#!/opt/chipster/tools/Python-2.7.12/bin/python # Time-stamp: <2009-11-08 20:03:20 Tao Liu> import os import sys # ------------------------------------ # Main function # ------------------------------------ def main(): if len(sys.argv) < 3: sys.stderr.write("Filter eland_multi.txt file and only keep the unique matches\nneed 2 paras: %s \n" % sys.argv[0]) sys.exit(1) eland_multi_fhd = file(sys.argv[1],"r") bed_fhd = file(sys.argv[2],"w") i = 0 m = 0 for thisline in eland_multi_fhd: __fw_parse_line(thisline,bed_fhd) i+=1 if i == 1000000: m += 1 sys.stderr.write(" %d\n" % (m*1000000)) i=0 def __fw_parse_line (thisline, fhd ): if not thisline: return (None,None,None) thisline = thisline.rstrip() if not thisline: return ("blank",None,None) if thisline[0] == "#": return ("comment line",None,None) # comment line is skipped thisfields = thisline.split() thistagname = thisfields[0] # name of tag thistaglength = len(thisfields[1]) # length of tag if len(thisfields) < 4: return (None,None,None) else: thistaghits = sum(map(int,thisfields[2].split(':'))) if thistaghits > 1: # multiple hits return (None,None,None) else: (name,pos) = thisfields[3].split(':') strand = pos[-2] if strand == "F": fhd.write( "%s\t%d\t%d\t.\t.\t%s\n" % (name, int(pos[:-2])-1, int(pos[:-2])+thistaglength-1, "+") ) elif strand == "R": fhd.write( "%s\t%d\t%d\t.\t.\t%s\n" % (name, int(pos[:-2])-1, int(pos[:-2])+thistaglength-1, "-") ) else: raise StrandFormatError(thisline,strand) class StrandFormatError(Exception): def __init__ (self, string, strand): self.message = "Strand information can not be recognized in this line: \"%s\",\"%s\"" % (string,strand) def __str__ (self): return repr(self.message) if __name__ == '__main__': main()