#include #include #include #include #include "bam.h" #ifdef _USE_KNETFILE #include "knetfile.h" #endif int bam_taf2baf(int argc, char *argv[]); int bam_mpileup(int argc, char *argv[]); int bam_merge(int argc, char *argv[]); int bam_index(int argc, char *argv[]); int bam_sort(int argc, char *argv[]); int bam_tview_main(int argc, char *argv[]); int bam_mating(int argc, char *argv[]); int bam_rmdup(int argc, char *argv[]); int bam_flagstat(int argc, char *argv[]); int bam_fillmd(int argc, char *argv[]); int bam_idxstats(int argc, char *argv[]); int main_samview(int argc, char *argv[]); int main_import(int argc, char *argv[]); int main_reheader(int argc, char *argv[]); int main_cut_target(int argc, char *argv[]); int main_phase(int argc, char *argv[]); int main_cat(int argc, char *argv[]); int main_depth(int argc, char *argv[]); int main_bam2fq(int argc, char *argv[]); int main_pad2unpad(int argc, char *argv[]); int main_bedcov(int argc, char *argv[]); int main_bamshuf(int argc, char *argv[]); int faidx_main(int argc, char *argv[]); static int usage() { fprintf(stderr, "\n"); fprintf(stderr, "Program: samtools (Tools for alignments in the SAM format)\n"); fprintf(stderr, "Version: %s\n\n", BAM_VERSION); fprintf(stderr, "Usage: samtools [options]\n\n"); fprintf(stderr, "Command: view SAM<->BAM conversion\n"); fprintf(stderr, " sort sort alignment file\n"); fprintf(stderr, " mpileup multi-way pileup\n"); fprintf(stderr, " depth compute the depth\n"); fprintf(stderr, " faidx index/extract FASTA\n"); #if _CURSES_LIB != 0 fprintf(stderr, " tview text alignment viewer\n"); #endif fprintf(stderr, " index index alignment\n"); fprintf(stderr, " idxstats BAM index stats (r595 or later)\n"); fprintf(stderr, " fixmate fix mate information\n"); fprintf(stderr, " flagstat simple stats\n"); fprintf(stderr, " calmd recalculate MD/NM tags and '=' bases\n"); fprintf(stderr, " merge merge sorted alignments\n"); fprintf(stderr, " rmdup remove PCR duplicates\n"); fprintf(stderr, " reheader replace BAM header\n"); fprintf(stderr, " cat concatenate BAMs\n"); fprintf(stderr, " bedcov read depth per BED region\n"); fprintf(stderr, " targetcut cut fosmid regions (for fosmid pool only)\n"); fprintf(stderr, " phase phase heterozygotes\n"); fprintf(stderr, " bamshuf shuffle and group alignments by name\n"); // fprintf(stderr, " depad convert padded BAM to unpadded BAM\n"); // not stable fprintf(stderr, "\n"); #ifdef _WIN32 fprintf(stderr, "\ Note: The Windows version of SAMtools is mainly designed for read-only\n\ operations, such as viewing the alignments and generating the pileup.\n\ Binary files generated by the Windows version may be buggy.\n\n"); #endif return 1; } int main(int argc, char *argv[]) { #ifdef _WIN32 setmode(fileno(stdout), O_BINARY); setmode(fileno(stdin), O_BINARY); #ifdef _USE_KNETFILE knet_win32_init(); #endif #endif if (argc < 2) return usage(); if (strcmp(argv[1], "view") == 0) return main_samview(argc-1, argv+1); else if (strcmp(argv[1], "import") == 0) return main_import(argc-1, argv+1); else if (strcmp(argv[1], "mpileup") == 0) return bam_mpileup(argc-1, argv+1); else if (strcmp(argv[1], "merge") == 0) return bam_merge(argc-1, argv+1); else if (strcmp(argv[1], "sort") == 0) return bam_sort(argc-1, argv+1); else if (strcmp(argv[1], "index") == 0) return bam_index(argc-1, argv+1); else if (strcmp(argv[1], "idxstats") == 0) return bam_idxstats(argc-1, argv+1); else if (strcmp(argv[1], "faidx") == 0) return faidx_main(argc-1, argv+1); else if (strcmp(argv[1], "fixmate") == 0) return bam_mating(argc-1, argv+1); else if (strcmp(argv[1], "rmdup") == 0) return bam_rmdup(argc-1, argv+1); else if (strcmp(argv[1], "flagstat") == 0) return bam_flagstat(argc-1, argv+1); else if (strcmp(argv[1], "calmd") == 0) return bam_fillmd(argc-1, argv+1); else if (strcmp(argv[1], "fillmd") == 0) return bam_fillmd(argc-1, argv+1); else if (strcmp(argv[1], "reheader") == 0) return main_reheader(argc-1, argv+1); else if (strcmp(argv[1], "cat") == 0) return main_cat(argc-1, argv+1); else if (strcmp(argv[1], "targetcut") == 0) return main_cut_target(argc-1, argv+1); else if (strcmp(argv[1], "phase") == 0) return main_phase(argc-1, argv+1); else if (strcmp(argv[1], "depth") == 0) return main_depth(argc-1, argv+1); else if (strcmp(argv[1], "bam2fq") == 0) return main_bam2fq(argc-1, argv+1); else if (strcmp(argv[1], "pad2unpad") == 0) return main_pad2unpad(argc-1, argv+1); else if (strcmp(argv[1], "depad") == 0) return main_pad2unpad(argc-1, argv+1); else if (strcmp(argv[1], "bedcov") == 0) return main_bedcov(argc-1, argv+1); else if (strcmp(argv[1], "bamshuf") == 0) return main_bamshuf(argc-1, argv+1); else if (strcmp(argv[1], "pileup") == 0) { fprintf(stderr, "[main] The `pileup' command has been removed. Please use `mpileup' instead.\n"); return 1; } #if _CURSES_LIB != 0 else if (strcmp(argv[1], "tview") == 0) return bam_tview_main(argc-1, argv+1); #endif else { fprintf(stderr, "[main] unrecognized command '%s'\n", argv[1]); return 1; } return 0; }