#include #include #include "faidx.h" #include "sam.h" #define TYPE_BAM 1 #define TYPE_READ 2 bam_header_t *bam_header_dup(const bam_header_t *h0) { bam_header_t *h; int i; h = bam_header_init(); *h = *h0; h->hash = h->dict = h->rg2lib = 0; h->text = (char*)calloc(h->l_text + 1, 1); memcpy(h->text, h0->text, h->l_text); h->target_len = (uint32_t*)calloc(h->n_targets, 4); h->target_name = (char**)calloc(h->n_targets, sizeof(void*)); for (i = 0; i < h->n_targets; ++i) { h->target_len[i] = h0->target_len[i]; h->target_name[i] = strdup(h0->target_name[i]); } return h; } static void append_header_text(bam_header_t *header, char* text, int len) { int x = header->l_text + 1; int y = header->l_text + len + 1; // 1 byte null if (text == 0) return; kroundup32(x); kroundup32(y); if (x < y) header->text = (char*)realloc(header->text, y); strncpy(header->text + header->l_text, text, len); // we cannot use strcpy() here. header->l_text += len; header->text[header->l_text] = 0; } int samthreads(samfile_t *fp, int n_threads, int n_sub_blks) { if (!(fp->type&1) || (fp->type&2)) return -1; bgzf_mt(fp->x.bam, n_threads, n_sub_blks); return 0; } samfile_t *samopen(const char *fn, const char *mode, const void *aux) { samfile_t *fp; fp = (samfile_t*)calloc(1, sizeof(samfile_t)); if (strchr(mode, 'r')) { // read fp->type |= TYPE_READ; if (strchr(mode, 'b')) { // binary fp->type |= TYPE_BAM; fp->x.bam = strcmp(fn, "-")? bam_open(fn, "r") : bam_dopen(fileno(stdin), "r"); if (fp->x.bam == 0) goto open_err_ret; fp->header = bam_header_read(fp->x.bam); } else { // text fp->x.tamr = sam_open(fn); if (fp->x.tamr == 0) goto open_err_ret; fp->header = sam_header_read(fp->x.tamr); if (fp->header->n_targets == 0) { // no @SQ fields if (aux) { // check if aux is present bam_header_t *textheader = fp->header; fp->header = sam_header_read2((const char*)aux); if (fp->header == 0) goto open_err_ret; append_header_text(fp->header, textheader->text, textheader->l_text); bam_header_destroy(textheader); } if (fp->header->n_targets == 0 && bam_verbose >= 1) fprintf(stderr, "[samopen] no @SQ lines in the header.\n"); } else if (bam_verbose >= 2) fprintf(stderr, "[samopen] SAM header is present: %d sequences.\n", fp->header->n_targets); } } else if (strchr(mode, 'w')) { // write fp->header = bam_header_dup((const bam_header_t*)aux); if (strchr(mode, 'b')) { // binary char bmode[3]; int i, compress_level = -1; for (i = 0; mode[i]; ++i) if (mode[i] >= '0' && mode[i] <= '9') break; if (mode[i]) compress_level = mode[i] - '0'; if (strchr(mode, 'u')) compress_level = 0; bmode[0] = 'w'; bmode[1] = compress_level < 0? 0 : compress_level + '0'; bmode[2] = 0; fp->type |= TYPE_BAM; fp->x.bam = strcmp(fn, "-")? bam_open(fn, bmode) : bam_dopen(fileno(stdout), bmode); if (fp->x.bam == 0) goto open_err_ret; bam_header_write(fp->x.bam, fp->header); } else { // text // open file fp->x.tamw = strcmp(fn, "-")? fopen(fn, "w") : stdout; if (fp->x.tamw == 0) goto open_err_ret; if (strchr(mode, 'X')) fp->type |= BAM_OFSTR<<2; else if (strchr(mode, 'x')) fp->type |= BAM_OFHEX<<2; else fp->type |= BAM_OFDEC<<2; // write header if (strchr(mode, 'h')) { int i; bam_header_t *alt; // parse the header text alt = bam_header_init(); alt->l_text = fp->header->l_text; alt->text = fp->header->text; sam_header_parse(alt); alt->l_text = 0; alt->text = 0; // check if there are @SQ lines in the header fwrite(fp->header->text, 1, fp->header->l_text, fp->x.tamw); // FIXME: better to skip the trailing NULL if (alt->n_targets) { // then write the header text without dumping ->target_{name,len} if (alt->n_targets != fp->header->n_targets && bam_verbose >= 1) fprintf(stderr, "[samopen] inconsistent number of target sequences. Output the text header.\n"); } else { // then dump ->target_{name,len} for (i = 0; i < fp->header->n_targets; ++i) fprintf(fp->x.tamw, "@SQ\tSN:%s\tLN:%d\n", fp->header->target_name[i], fp->header->target_len[i]); } bam_header_destroy(alt); } } } return fp; open_err_ret: free(fp); return 0; } void samclose(samfile_t *fp) { if (fp == 0) return; if (fp->header) bam_header_destroy(fp->header); if (fp->type & TYPE_BAM) bam_close(fp->x.bam); else if (fp->type & TYPE_READ) sam_close(fp->x.tamr); else fclose(fp->x.tamw); free(fp); } int samread(samfile_t *fp, bam1_t *b) { if (fp == 0 || !(fp->type & TYPE_READ)) return -1; // not open for reading if (fp->type & TYPE_BAM) return bam_read1(fp->x.bam, b); else return sam_read1(fp->x.tamr, fp->header, b); } int samwrite(samfile_t *fp, const bam1_t *b) { if (fp == 0 || (fp->type & TYPE_READ)) return -1; // not open for writing if (fp->type & TYPE_BAM) return bam_write1(fp->x.bam, b); else { char *s = bam_format1_core(fp->header, b, fp->type>>2&3); int l = strlen(s); fputs(s, fp->x.tamw); fputc('\n', fp->x.tamw); free(s); return l + 1; } } int sampileup(samfile_t *fp, int mask, bam_pileup_f func, void *func_data) { bam_plbuf_t *buf; int ret; bam1_t *b; b = bam_init1(); buf = bam_plbuf_init(func, func_data); bam_plbuf_set_mask(buf, mask); while ((ret = samread(fp, b)) >= 0) bam_plbuf_push(b, buf); bam_plbuf_push(0, buf); bam_plbuf_destroy(buf); bam_destroy1(b); return 0; } char *samfaipath(const char *fn_ref) { char *fn_list = 0; if (fn_ref == 0) return 0; fn_list = calloc(strlen(fn_ref) + 5, 1); strcat(strcpy(fn_list, fn_ref), ".fai"); if (access(fn_list, R_OK) == -1) { // fn_list is unreadable if (access(fn_ref, R_OK) == -1) { fprintf(stderr, "[samfaipath] fail to read file %s.\n", fn_ref); } else { if (bam_verbose >= 3) fprintf(stderr, "[samfaipath] build FASTA index...\n"); if (fai_build(fn_ref) == -1) { fprintf(stderr, "[samfaipath] fail to build FASTA index.\n"); free(fn_list); fn_list = 0; } } } return fn_list; }