#include #include #include #include #include #ifdef _WIN32 #define drand48() ((double)rand() / RAND_MAX) #endif #include "ksort.h" KSORT_INIT_GENERIC(uint64_t) #include "kseq.h" KSTREAM_INIT(gzFile, gzread, 8192) typedef struct { int n, m; uint64_t *a; int *idx; } bed_reglist_t; #include "khash.h" KHASH_MAP_INIT_STR(reg, bed_reglist_t) #define LIDX_SHIFT 13 typedef kh_reg_t reghash_t; int *bed_index_core(int n, uint64_t *a, int *n_idx) { int i, j, m, *idx; m = *n_idx = 0; idx = 0; for (i = 0; i < n; ++i) { int beg, end; beg = a[i]>>32 >> LIDX_SHIFT; end = ((uint32_t)a[i]) >> LIDX_SHIFT; if (m < end + 1) { int oldm = m; m = end + 1; kroundup32(m); idx = realloc(idx, m * sizeof(int)); for (j = oldm; j < m; ++j) idx[j] = -1; } if (beg == end) { if (idx[beg] < 0) idx[beg] = i; } else { for (j = beg; j <= end; ++j) if (idx[j] < 0) idx[j] = i; } *n_idx = end + 1; } return idx; } void bed_index(void *_h) { reghash_t *h = (reghash_t*)_h; khint_t k; for (k = 0; k < kh_end(h); ++k) { if (kh_exist(h, k)) { bed_reglist_t *p = &kh_val(h, k); if (p->idx) free(p->idx); ks_introsort(uint64_t, p->n, p->a); p->idx = bed_index_core(p->n, p->a, &p->m); } } } int bed_overlap_core(const bed_reglist_t *p, int beg, int end) { int i, min_off; if (p->n == 0) return 0; min_off = (beg>>LIDX_SHIFT >= p->n)? p->idx[p->n-1] : p->idx[beg>>LIDX_SHIFT]; if (min_off < 0) { // TODO: this block can be improved, but speed should not matter too much here int n = beg>>LIDX_SHIFT; if (n > p->n) n = p->n; for (i = n - 1; i >= 0; --i) if (p->idx[i] >= 0) break; min_off = i >= 0? p->idx[i] : 0; } for (i = min_off; i < p->n; ++i) { if ((int)(p->a[i]>>32) >= end) break; // out of range; no need to proceed if ((int32_t)p->a[i] > beg && (int32_t)(p->a[i]>>32) < end) return 1; // find the overlap; return } return 0; } int bed_overlap(const void *_h, const char *chr, int beg, int end) { const reghash_t *h = (const reghash_t*)_h; khint_t k; if (!h) return 0; k = kh_get(reg, h, chr); if (k == kh_end(h)) return 0; return bed_overlap_core(&kh_val(h, k), beg, end); } void *bed_read(const char *fn) { reghash_t *h = kh_init(reg); gzFile fp; kstream_t *ks; int dret; kstring_t *str; // read the list fp = strcmp(fn, "-")? gzopen(fn, "r") : gzdopen(fileno(stdin), "r"); if (fp == 0) return 0; str = calloc(1, sizeof(kstring_t)); ks = ks_init(fp); while (ks_getuntil(ks, 0, str, &dret) >= 0) { // read the chr name int beg = -1, end = -1; bed_reglist_t *p; khint_t k = kh_get(reg, h, str->s); if (k == kh_end(h)) { // absent from the hash table int ret; char *s = strdup(str->s); k = kh_put(reg, h, s, &ret); memset(&kh_val(h, k), 0, sizeof(bed_reglist_t)); } p = &kh_val(h, k); if (dret != '\n') { // if the lines has other characters if (ks_getuntil(ks, 0, str, &dret) > 0 && isdigit(str->s[0])) { beg = atoi(str->s); // begin if (dret != '\n') { if (ks_getuntil(ks, 0, str, &dret) > 0 && isdigit(str->s[0])) { end = atoi(str->s); // end if (end < beg) end = -1; } } } } if (dret != '\n') while ((dret = ks_getc(ks)) > 0 && dret != '\n'); // skip the rest of the line if (end < 0 && beg > 0) end = beg, beg = beg - 1; // if there is only one column if (beg >= 0 && end > beg) { if (p->n == p->m) { p->m = p->m? p->m<<1 : 4; p->a = realloc(p->a, p->m * 8); } p->a[p->n++] = (uint64_t)beg<<32 | end; } } ks_destroy(ks); gzclose(fp); free(str->s); free(str); bed_index(h); return h; } void bed_destroy(void *_h) { reghash_t *h = (reghash_t*)_h; khint_t k; for (k = 0; k < kh_end(h); ++k) { if (kh_exist(h, k)) { free(kh_val(h, k).a); free(kh_val(h, k).idx); free((char*)kh_key(h, k)); } } kh_destroy(reg, h); }