DESeq Differential expression analysis based on the Negative Binomial (a.k.a. Gamma-Poisson) distribution DESeq2-package DESeq2 package for differential analysis of count data DESeqDataSet-class DESeqDataSet object and constructors DESeqResults-class DESeqResults object and constructor DESeqTransform-class DESeqTransform object and constructor coef Extract a matrix of model coefficients/standard errors collapseReplicates Collapse technical replicates in a RangedSummarizedExperiment or DESeqDataSet counts Accessors for the 'counts' slot of a DESeqDataSet object. design Accessors for the 'design' slot of a DESeqDataSet object. dispersionFunction Accessors for the 'dispersionFunction' slot of a DESeqDataSet object. dispersions Accessor functions for the dispersion estimates in a DESeqDataSet object. estimateBetaPriorVar Steps for estimating the beta prior variance estimateDispersions Estimate the dispersions for a DESeqDataSet estimateDispersionsGeneEst Low-level functions to fit dispersion estimates estimateSizeFactors Estimate the size factors for a DESeqDataSet estimateSizeFactorsForMatrix Low-level function to estimate size factors with robust regression. fpkm FPKM: fragments per kilobase per million mapped fragments fpm FPM: fragments per million mapped fragments makeExampleDESeqDataSet Make a simulated DESeqDataSet nbinomLRT Likelihood ratio test (chi-squared test) for GLMs nbinomWaldTest Wald test for the GLM coefficients normTransform Normalized counts transformation normalizationFactors Accessor functions for the normalization factors in a DESeqDataSet object. normalizeGeneLength Normalize for gene length plotCounts Plot of normalized counts for a single gene on log scale plotDispEsts Plot dispersion estimates plotMA MA-plot from base means and log fold changes plotPCA Sample PCA plot for transformed data plotSparsity Sparsity plot replaceOutliers Replace outliers with trimmed mean results Extract results from a DESeq analysis rlog Apply a 'regularized log' transformation show Show method for DESeqResults objects sizeFactors Accessor functions for the 'sizeFactors' information in a DESeqDataSet object. summary Summarize DESeq results varianceStabilizingTransformation Apply a variance stabilizing transformation (VST) to the count data