#include #include #include "kstring.h" #include "utils.h" #ifndef PACKAGE_VERSION #define PACKAGE_VERSION "0.7.12-r1039" #endif int bwa_fa2pac(int argc, char *argv[]); int bwa_pac2bwt(int argc, char *argv[]); int bwa_bwtupdate(int argc, char *argv[]); int bwa_bwt2sa(int argc, char *argv[]); int bwa_index(int argc, char *argv[]); int bwt_bwtgen_main(int argc, char *argv[]); int bwa_aln(int argc, char *argv[]); int bwa_sai2sam_se(int argc, char *argv[]); int bwa_sai2sam_pe(int argc, char *argv[]); int bwa_bwtsw2(int argc, char *argv[]); int main_fastmap(int argc, char *argv[]); int main_mem(int argc, char *argv[]); int main_shm(int argc, char *argv[]); int main_pemerge(int argc, char *argv[]); static int usage() { fprintf(stderr, "\n"); fprintf(stderr, "Program: bwa (alignment via Burrows-Wheeler transformation)\n"); fprintf(stderr, "Version: %s\n", PACKAGE_VERSION); fprintf(stderr, "Contact: Heng Li \n\n"); fprintf(stderr, "Usage: bwa [options]\n\n"); fprintf(stderr, "Command: index index sequences in the FASTA format\n"); fprintf(stderr, " mem BWA-MEM algorithm\n"); fprintf(stderr, " fastmap identify super-maximal exact matches\n"); fprintf(stderr, " pemerge merge overlapping paired ends (EXPERIMENTAL)\n"); fprintf(stderr, " aln gapped/ungapped alignment\n"); fprintf(stderr, " samse generate alignment (single ended)\n"); fprintf(stderr, " sampe generate alignment (paired ended)\n"); fprintf(stderr, " bwasw BWA-SW for long queries\n"); fprintf(stderr, "\n"); fprintf(stderr, " shm manage indices in shared memory\n"); fprintf(stderr, " fa2pac convert FASTA to PAC format\n"); fprintf(stderr, " pac2bwt generate BWT from PAC\n"); fprintf(stderr, " pac2bwtgen alternative algorithm for generating BWT\n"); fprintf(stderr, " bwtupdate update .bwt to the new format\n"); fprintf(stderr, " bwt2sa generate SA from BWT and Occ\n"); fprintf(stderr, "\n"); fprintf(stderr, "Note: To use BWA, you need to first index the genome with `bwa index'.\n" " There are three alignment algorithms in BWA: `mem', `bwasw', and\n" " `aln/samse/sampe'. If you are not sure which to use, try `bwa mem'\n" " first. Please `man ./bwa.1' for the manual.\n\n"); return 1; } int main(int argc, char *argv[]) { extern char *bwa_pg; int i, ret; double t_real; kstring_t pg = {0,0,0}; t_real = realtime(); ksprintf(&pg, "@PG\tID:bwa\tPN:bwa\tVN:%s\tCL:%s", PACKAGE_VERSION, argv[0]); for (i = 1; i < argc; ++i) ksprintf(&pg, " %s", argv[i]); bwa_pg = pg.s; if (argc < 2) return usage(); if (strcmp(argv[1], "fa2pac") == 0) ret = bwa_fa2pac(argc-1, argv+1); else if (strcmp(argv[1], "pac2bwt") == 0) ret = bwa_pac2bwt(argc-1, argv+1); else if (strcmp(argv[1], "pac2bwtgen") == 0) ret = bwt_bwtgen_main(argc-1, argv+1); else if (strcmp(argv[1], "bwtupdate") == 0) ret = bwa_bwtupdate(argc-1, argv+1); else if (strcmp(argv[1], "bwt2sa") == 0) ret = bwa_bwt2sa(argc-1, argv+1); else if (strcmp(argv[1], "index") == 0) ret = bwa_index(argc-1, argv+1); else if (strcmp(argv[1], "aln") == 0) ret = bwa_aln(argc-1, argv+1); else if (strcmp(argv[1], "samse") == 0) ret = bwa_sai2sam_se(argc-1, argv+1); else if (strcmp(argv[1], "sampe") == 0) ret = bwa_sai2sam_pe(argc-1, argv+1); else if (strcmp(argv[1], "bwtsw2") == 0) ret = bwa_bwtsw2(argc-1, argv+1); else if (strcmp(argv[1], "dbwtsw") == 0) ret = bwa_bwtsw2(argc-1, argv+1); else if (strcmp(argv[1], "bwasw") == 0) ret = bwa_bwtsw2(argc-1, argv+1); else if (strcmp(argv[1], "fastmap") == 0) ret = main_fastmap(argc-1, argv+1); else if (strcmp(argv[1], "mem") == 0) ret = main_mem(argc-1, argv+1); else if (strcmp(argv[1], "shm") == 0) ret = main_shm(argc-1, argv+1); else if (strcmp(argv[1], "pemerge") == 0) ret = main_pemerge(argc-1, argv+1); else { fprintf(stderr, "[main] unrecognized command '%s'\n", argv[1]); return 1; } err_fflush(stdout); err_fclose(stdout); if (ret == 0) { fprintf(stderr, "[%s] Version: %s\n", __func__, PACKAGE_VERSION); fprintf(stderr, "[%s] CMD:", __func__); for (i = 0; i < argc; ++i) fprintf(stderr, " %s", argv[i]); fprintf(stderr, "\n[%s] Real time: %.3f sec; CPU: %.3f sec\n", __func__, realtime() - t_real, cputime()); } free(bwa_pg); return ret; }