#ifndef BAM2BCF_H #define BAM2BCF_H #include #include "errmod.h" #include "bcftools/bcf.h" #define B2B_INDEL_NULL 10000 #define B2B_FMT_DP 0x1 #define B2B_FMT_SP 0x2 #define B2B_FMT_DV 0x4 typedef struct __bcf_callaux_t { int capQ, min_baseQ; int openQ, extQ, tandemQ; // for indels int min_support, max_support; // for collecting indel candidates double min_frac, max_frac; // for collecting indel candidates int per_sample_flt; // indel filtering strategy int *ref_pos, *alt_pos, npos; // for ReadPosBias // for internal uses int max_bases; int indel_types[4]; int maxins, indelreg; int read_len; char *inscns; uint16_t *bases; errmod_t *e; void *rghash; } bcf_callaux_t; typedef struct { int depth, n_supp, ori_depth, qsum[4]; unsigned int anno[16]; float p[25]; } bcf_callret1_t; typedef struct { int a[5]; // alleles: ref, alt, alt2, alt3 float qsum[4]; int n, n_alleles, shift, ori_ref, unseen; int n_supp; // number of supporting non-reference reads unsigned int anno[16], depth, ori_depth; uint8_t *PL; float vdb; // variant distance bias float read_pos_bias; struct { float avg, var; int dp; } read_pos; } bcf_call_t; #ifdef __cplusplus extern "C" { #endif bcf_callaux_t *bcf_call_init(double theta, int min_baseQ); void bcf_call_destroy(bcf_callaux_t *bca); int bcf_call_glfgen(int _n, const bam_pileup1_t *pl, int ref_base, bcf_callaux_t *bca, bcf_callret1_t *r); int bcf_call_combine(int n, const bcf_callret1_t *calls, bcf_callaux_t *bca, int ref_base /*4-bit*/, bcf_call_t *call); int bcf_call2bcf(int tid, int pos, bcf_call_t *bc, bcf1_t *b, bcf_callret1_t *bcr, int fmt_flag, const bcf_callaux_t *bca, const char *ref); int bcf_call_gap_prep(int n, int *n_plp, bam_pileup1_t **plp, int pos, bcf_callaux_t *bca, const char *ref, const void *rghash); #ifdef __cplusplus } #endif #endif