PMID- 16929101 OWN - NLM STAT- MEDLINE DA - 20060824 DCOM- 20070116 LR - 20070724 IS - 0907-4449 (Print) IS - 0907-4449 (Linking) VI - 62 IP - Pt 9 DP - 2006 Sep TI - The Buccaneer software for automated model building. 1. Tracing protein chains. PG - 1002-11 AB - A new technique for the automated tracing of protein chains in experimental electron-density maps is described. The technique relies on the repeated application of an oriented electron-density likelihood target function to identify likely C(alpha) positions. This function is applied both in the location of a few promising ;seed' positions in the map and to grow those initial C(alpha) positions into extended chain fragments. Techniques for assembling the chain fragments into an initial chain trace are discussed. FAU - Cowtan, Kevin AU - Cowtan K AD - Department of Chemistry, University of York, Heslington, York YO10 5DD, England. cowtan@ysbl.york.ac.uk LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20060819 PL - Denmark TA - Acta Crystallogr D Biol Crystallogr JT - Acta crystallographica. Section D, Biological crystallography JID - 9305878 RN - 0 (Proteins) RN - EC 1.- (Mixed Function Oxygenases) RN - EC 1.14.11.- (aspartic acid 2-oxoglutarate-dependent dioxygenase) SB - IM MH - Algorithms MH - Automation MH - Bacillus subtilis/enzymology MH - Computational Biology/*methods MH - Crystallography, X-Ray MH - Mixed Function Oxygenases/chemistry MH - Models, Molecular MH - Models, Statistical MH - Models, Theoretical MH - Molecular Conformation MH - Probability MH - Proteins/*chemistry MH - Proteomics/methods MH - *Software EDAT- 2006/08/25 09:00 MHDA- 2007/01/17 09:00 CRDT- 2006/08/25 09:00 PHST- 2006/08/19 [epublish] AID - S0907444906022116 [pii] AID - 10.1107/S0907444906022116 [doi] PST - ppublish SO - Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):1002-11. Epub 2006 Aug 19.