Module: Perfusion Analysis ()
Computes mean transit time (MTT), blood flow (CBF), and blood volume (CBV) from a perfusion weighted image series (MR or CT). The different computations for CT or MRI images are selected based on the DICOM tag echo time (0018,0081). For MR data this tag should contain a value larger than 0.Load your (DICOM) images as a time series and connect this module to the TimeSeriesControl module. The module requires a spreadsheet as secondary input. This table can be created by the module using the sample button functionality. Press a sample button (e.g., AIF) and in interactive mode select a vessel for arterial inflow or venous outflow using the middle mouse button. A spreadsheet object should be created which contains the sampled time-density curve at that location.
The algorithm implemented in this module performs the perfusion computation based on deconvolution.
Data [required]
Connect this port to a TimeSeriesControl module. The connected time series will be used for computation.AIF [optional]
This port can be connected to a SpreadSheet object. The spreadsheet should contain a single float-valued column "AIF" (arterial input function) with a sample for each time point in the series. A second column "VOF" can be provided for the venous output function.Mask [optional]
If a mask data set is connected, the computation is restricted to areas that are not marked as Exterior.
Sample
The sample port provides two functions, one for sampling the arterial input and one for the venous output function. The data should be visualized using an OrthoSlice module (or similar) to provide the point of interest in 3D space. Press one of the buttons and, in the interactive mode (cursor shows arrow), select a point on the slice using the middle mouse button. This 3D location is used as a seed point for a search algorithm that looks for the voxels with the strongest signal within a radius specified in port Search Radius. An average over several of the voxels is used to calculate the time-density function from the connected time series. The result is stored in a SpreadSheet object connected to the AIF port of this module.The voxel positions used for the computation of the input and output functions can be stored in a LabelField. The creation of such a LabelField can be enabled with the Tcl command createLabelfield 1. It includes 5 materials: "Exterior", "AIF_search_area", "AIF_measurement", "VOF_search_area" und "VOF_measurement"
Time and Phase
Phases in Baseline [advanced]
The number of images used to compute the pre-contrast baseline. Adjust this value if less than the default number of phases before contrast adaptation are recorded.Note that for MRI data the first image will always be removed from the computation as it usually contains an artificially high signal intensity.
Limit to phase [advanced]
With this port the computation can be restricted to a number of volumes smaller than the full recorded time series. In some cases, this can reduce the effect of noise on the computation and limit the effect of recirculation on the computation.Inter-frame time [advanced]
This port specifies the time between image frames in seconds.
Physiology Parameters
Density Factor [advanced]
This value describes the density of brain tissue. The value should not be changed for normal operation.Hematocrit Large Vessel
A ratio which is used by the algorithm. The value should not be changed for normal operation.Hematocrit Small Vessel
A ratio which is used by the algorithm. The value should not be changed for normal operation.
Algorithm Parameters
Tikhonov Delta [advanced]
This value sets a regularization factor used during the deconvolution step. As a matrix inversion is required, this regularization attempts to reduce the influence of noise on the result of the reconstruction. Use a lower value to regularize more.Signal-to-noise [advanced]
A warning message is produced if the signal-to-noise ratio of the images in the baseline (before contrast) is smaller than this value. In case this warning is produced, the value of the phases in baseline should be adapted to not include the initial inflow of contrast. The warning might also be produced if the data contains more than the normal amount of noise.Search Radius [advanced]
This port specifies the radius in mm around the seed point that is searched for optimum signal strength of the AIF and VOF. Note, the measurement unit is only in mm if the voxel size or bounding box of the data set is specified in mm.
Smoothing
Smoothing [advanced]
Activates Gaussian smoothing.Kernel Size [advanced]
Specifies the size of the Gaussian filter kernel.Smoothing Kernel Sigma [advanced]
Specifies the sigma of the Gaussian filter kernel.
Filter Voxels
Filter Voxel [advanced]
This port enables averaging of the artirial input and venous output functions.Minimum Uptake Difference [advanced]
The minimum uptake difference can be adjusted, which is required to use a voxel for the AIF/VOF calculation. The difference is calculated between voxel values at the first time point and all consecutive time points.Maximum Start Value [advanced]
The maximum value that a voxel of the AIF/VOF can have to be a valid seed point.