Phaser Crystallographic Software

From Phaserwiki

Phaser is a program for phasing macromolecular crystal structures with maximum likelihood methods. It has been developed by Randy Read's group at the Cambridge Institute for Medical Research (CIMR) in the University of Cambridge and is available through the Phenix and CCP4 software suites.

Use the sidebar to navigate through the extensive documentation for Phaser.

This PhaserWiki supersedes the obsolete Phaser homepage, which now redirects to this wiki. A copy of the obsolete Phaser homepage can be found here

Currently Supported Releases

Phaser-2.7.14

Download with Phenix Nightly Builds → Phenix
Download with CCP4 Nightly Builds → CCP4
Documentation → Manual

Changes

  • SOLUTION HISTORY tracks solution through positions in RF/TF/PAK/RNP peak lists
  • selection by CHAIN and MODEL for PDB coordinate entry
  • automatic search number for single search ensemble
  • packing 'trace' molecule can be entered independently of coordinates and map
  • read TNCS/anisotropy binary files to avoid refinement, when running through scripts
  • write tNCS and anisotropy parameters to binary files (non-python interface)
  • default reading of I (or failing that, F) from mtz file (LABIN optional)
  • trace for ensembles from maps = hexgrid of 1000+/-100 points
  • trace for ensembles from coordinates above 1000 Calpha = hexgrid of 1000+/-100 points
  • trace for ensembles from coordinates twixt 1000 atoms and 1000 Calpha = Calpha atoms
  • trace for ensembles from coordinates under 1000 atoms = all atoms
  • packing by pairwise percent only, other packing modes obsoleted
  • packing test during FTF run by default with 50% pairwise packing cutoff
  • automatic tNCS NMOL determination in presence of commensurate modulation
  • added MODE GIMBLE, which splits ensembles by chain for rigid body refinement
  • support for unicode
  • solution coordinates placed nearest to input coordinates if possible

Phaser-2.6.0

Download with Phenix Official Release 1.10 (September 2015) → Phenix
Download with CCP4 7.0 → CCP4
Documentation → Manual


Referencing Phaser

Citing crystallographic software in your paper is important for funding new software development. We rely on your citations to convince funding bodies that our software is being used.

If you solve a structure with Phaser, please cite

Phaser crystallographic software
McCoy AJ, Grosse-Kunstleve RW, Adams PD, Winn MD, Storoni LC, Read RJ.
J Appl Cryst (2007). 40, 658-674.